Reporter

Part:BBa_K3873999:Design

Designed by: Qinwen Wang   Group: iGEM21_NJMU-China   (2021-10-04)


An AND gate composed of a 36℃-induced promoter and Tryptophan riboswitch as a multi input modu


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 569
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 854
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1157
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Biotechnol Bioeng. 2018 Jan;115(1):266-271. doi: 10.1002/bit.26448. Epub 2017 Oct 6. Systematic optimization of L-tryptophan riboswitches for efficient monitoring of the metabolite in Escherichia coli

References